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KMID : 1189420210340040001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2021 Volume.34 No. 4 p.1 ~ p.11
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Skin Anti-aging and Anti-wrinkle Effects of Pinus koreaiensis Seed Oil
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Kim Hyung-Mook
Kim Tae-Jun
Im Dong-Bin
Ha Sun-Bong
Kim Ee-Hwa
Cha Byung-Sun
Heo Hyo-Jin
Sofia Brito
Lee Yong-Moon
Bin Bum-Ho
Kwak Byeong-Mun
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Abstract
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Objectives : The aim of this study was to investigated the anti-aging and anti-wrinkles effects of the pinus koreaiensis seed(PKS) oil.
Methods : The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. And fatty acid was analysed by gas chromatography. Anti-wrinkle effect was analyzing human skin by the PRIMOS system.
Results : Fatty acid analysis of PKS oil has shown oleic acid was 49.7% and linoleic acid was 34.1%. And the antioxidant effect was about 125% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. In case of anti-wrinkle effect was proved in vivo by analyzing human skin by the PRIMOS system. The analysis results of eye wrinkles for 4 weeks showed an improvement effect of over 6%.
Conclusions : In this study, the amount of protein change in the five mechanism through the cell experiment and the skin anti wrinkle efficacy by the human in vivo test were investigated. As a result pinus koreaiensis seed oil by supercritical extraction could be used as a anti-aging and anti-wrinkle substance for the skin.
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KEYWORD
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Pinus koreaiensis, Anti-oxidant, Anti-weinkle, Supercritical fluid extraction, Anti-oxidant
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